IJBST 2018 Volume 11 Issue 6
International Journal of BioSciences and Technology (IJBST) ISSN: 0974-3987
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Mathew John, Nimmy Mohandas, & Sinju R. (2018). Electrophoretic Protein Analysis of Red blood cell Membrane Proteins upon Ionic and Non-ionic detergent lysis. International Journal of Biosciences and Technology (IJBST) ISSN: 0974-3987, 11(6), 52–59. http://doi.org/10.5281/zenodo.1491957
Title:
Electrophoretic Protein Analysis of Red blood cell Membrane Proteins upon Ionic and Non-ionic detergent lysis
Authors & Affiliation
Mathew John1, Nimmy Mohandas2, Sinju R3
1, 2 JCMR Jubilee Mission Medical College & Research Institute, Thrissur - 680 005, Kerala, India
3 Department of Biochemistry, Jubilee Mission Medical College & Research Institute, Thrissur - 680 005, Kerala, India
mathewjohn@jmmc.ac.in, nimmymohandas6@gmail.com, sinjurajan7@gmail.com
Abstract:
Red blood cells (RBC) are anucleated atypical cellular architecture with a plasma membrane envelope where the glycoproteins are oriented along the lipid bilayer. RBC proteins are classified as peripheral and integral proteins based on its location along plasma membrane. The present study explored the extraction efficiency of various detergents such as Triton X100, Sodium dodecyl sulphate (SDS), Cetypyridinium chloride (CPC), alone and its combinations along with protease inhibitor cocktail for RBC membrane proteins analyzed in an elctrophoretogram. The study showed that RBC protein extraction was much better in combination of Triton X100 and SDS as evident by increased number of protein bands in coomassie brilliant blue R stained SDS gel, compared to CPC combined Triton X100 lysis buffer. The study hence showed that RBC proteins can be extracted with combination of non-ionic detergent and anionic detergent.
Key words: Red blood cells, Triton X100, Sodium dodecyl sulphate, Cetyl pyridinium chloride