IJBST 2017 Volume 10 Issue 3
International Journal of BioSciences and Technology (IJBST) ISSN: 0974-3987
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In vitro propagation of a threatened medicinal plant Satureja abyssinica through nodal explants – Antimicrobial and antifungal herb. Shiferaw Teshome, Teshome Soromessa, Tileye Feyissa. IJBST (2017), 10(3):20-25
FULL TEXT SWF:
Title:
In vitro propagation of a threatened medicinal plant Satureja abyssinica through nodal explants – Antimicrobial and antifungal herb
Authors & Affiliation:
Shiferaw Teshome1, Teshome Soromessa2, Tileye Feyissa3
1 Department of Biotechnology, Wolaita Sodo University, P.O. Box 138, Sodo, Ethiopia
2 Center for Environmental Science, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
1,3 Institute of Biotechnology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
3 Nelson Mandela African Institution of Science and Technology, P. O. Box 447, Arusha, Tanzania
tesheshife@gmail.com, teshome.soromessa@aau.edu.et, tileye_feyissa@yahoo.com
Abstract:
Satureja abyssinica (Benth.) Briq. is an endangered medical and aromatic herb that belongs to Lamiaceae (Labiateae) family and well known for its antimicrobial and antifungal properties. This species is highly threatened due to continuously collecting from the wild and absence of germplasm conservation techniques. In this study, direct organogenesis protocol has been developed for this species through nodal segment explants. MS medium containing different concentrations of 6-benzyl aminopurine (BAP), Kinetin (KIN), α-Naphthalene acetic acid (NAA), Indole-3- butyric acid (IBA) and indole acetic acid (IAA) were utilized in this experiment. Best culture establishment (90%), maximum average of shoot (3.30±0.47) and shoot length (2.35±0.28 cm) were observed on initiation medium containing 1.0 mg/l BAP. Multiple shoots with highest (3.90±0.60) of shoots per nodal segment and shoot length (1.10±0.16 cm) were obtained at 1.0 mg/l BAP. Maximum root number (8.00±1.12) and 90% rooting frequency were achieved on half-strength MS medium supplemented with 0.5 mg/l IBA. Mean of longest root (1.07±0.09 cm) was produced in the presence of 1.4 mg/l IBA. After acclimatization, 88% plant survived. This protocol can be used for rapid propagation and consequent improvement and conservation of S. abyssinica.
Keywords: Endangered; nodal explants; Satureja; shoot multiplication