Antibacterial Activity and Protein Sequences of Actinomycetes Isolated from Coastal Area of Niger Delta against Human and Fish Pathogens
Ebele L. Okoye , Bright O. Uba and Chidinma E. Onwunyili
IJBST (2020), 13(1):1-17
Ebele L. Okoye, Bright O. Uba, & Chidinma E. Onwunyili. (2020). Antibacterial Activity and Protein Sequences of Actinomycetes Isolated from Coastal Area of Niger Delta against Human and Fish Pathogens. International Journal of Biosciences and Technology (IJBST) ISSN: 0974-3987, 13(1), 1–17. http://doi.org/10.5281/zenodo.3617213
(Collapsible menu. Expands on click)
Actinomycetes are known to produce potential secondary metabolites which possess biological activity. This knowledge spurred the present study in which the antibacterial activity and protein sequences of Actinomycetes isolated from Coastal Area of Niger Delta against human and fish pathogens were evaluated. Marine sediment and water samples were collected from ten (10) different points from Onne light flow terminal seaport located in Eleme Local Government Area of Rivers State, Nigeria. A total of fifteen (15) Actinomycetes were isolated on Modified Glycerol Starch Casein Nitrate Agar using spread plate technique. All the isolates were characterized and identified using standard procedures. The API system of identification used revealed that thirteen of the isolates belong to the genus Streptomyces while two belong to the genus Actinomyces. The isolated marine Actinomycetes were screened for their antibacterial activities against four human bacterial pathogens namely:- Staphylococcus aureus, Escherichia coli, Salmonella Typhi and Shigella dysenteriae and four fish bacterial pathogens:- Staphylococcus aureus P1, Vibrio sp. MF2, Salmonella sp. and Vibrio sp. S1. The antibacterial screening revealed that amongst the fifteen (15) isolates, ten (10) showed inhibitory activities against the human pathogens while fourteen (14) strains showed inhibitory activities against the fish pathogens. The two most potent isolates were ATS-3 and ATS-1 which were both identified as Steptomyces lavendulae. ATS-3 showed the highest inhibitory activity against S. dysenteriae (30.00 ± 0.25 mm) and lowest activity against S. aureus (10.00 ± 0.16 mm) while ATS-1 strain showed highest inhibitory activity against Vibro sp. S1 (25.00 ± 5.20 mm) and lowest inhibitory activity against S. aureus P1 (11.00 ± 2.24 mm). Their inhibitory activities were statistically significant with P values less than 0.05 (P<0.05). The protein profiling of the partially purified whole cell proteins of ATS-3 and ATS-1 on SDS-PAGE exhibited apparent lower and upper molecular weights of 71.7 kDa and 47.5 kDa and 70.4 kDa and 41.16 kDa respectively. The result of the taxonomic correctional analysis revealed that the protein sequences of the two strains were closely related to each other with 80 % similarities suggesting that the strains belonged to the genus Streptomyces. Thus, the fact that majority of Actinomycetes strains (66.67 %) in the studied area produced potential bioactive compounds against the human and fish bacterial pathogens studied revealed that the marine actinomcycetes from the Onne light flow terminal seaport could serve as potential sources of novel antibiotics.
Keywords: Actinomycetes, Antibacterial activity, Fish pathogens, Human pathogens, Coastal area, Niger Delta.
The IJBST Journal Group Serves Free since Establishment in year 2008
IJBST Journal Group -- Open Access -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives
Free University / Institutional Subscription of the IJBST Journal Group https://subscription.approvals.ijbst.org
The IJBST Journal Group subscribes to the San Francisco Declaration on Research Assessment and The European Code of Conduct for Research Integrity and The ICMJE recommendations for the Conduct, Reporting, Editing, and Publication of Scholarly work
The IJBST Journal Group Archive can also be accessed at https://archive.org/details/IJBSTJournalGroup
Anniversary Message: IJBST Journal Group. January 2008 -- .....
(Collapsible menu. Expands on click)
It was in January 2008, that the IJBST Journal Group embarked on a Journey of providing a free open access platform, serving with ethics & integrity, without charging any fees, even for its sustenance.
In our pursuit of providing such a service with ethics and integrity, the journey made us walk through carpetted corridors of power, pleasantly surprised to find that those towering personalities of the planet had considered fondly, even our smallest contributions to the world.
We were bestowed unexpected respect, at the most high places of this planet.
Our efforts continued, without sacrificing our high ideals of free research publication service with ethics & integrity.
We are now embarking on another year of our free open access service with ethics & integrity, with the faith that the seeds that we have sown, shall yield good fruit for the world for all of mankind & nature, for years to come.
This day, we remember fondly our Emeritus Patrons (Late), and all those towering personalities who were with us in our Journey.
At this juncture, we record our humble and most sincere gratitude to our Patrons, Emeritus Patrons, Visitors, Presidium, Board, Authors, Subscribing Institutions, ROAD, NISCAIR, EBSCO, PROQUEST, CABI, ZENODO, Google, Microsoft & EUCED, and all those who stood with us & against us.
~ Prabhu Britto Albert, Founding Editor, IJBST Journal Group http://www.ijbst.org