IJBST 2017 Volume 10 Issue 11
International Journal of BioSciences and Technology (IJBST) ISSN: 0974-3987
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GC-MS Analysis and Phytochemical Screening of n-Hexane Fraction Constituents from the Leaf of Clerodendrum volubile P. Beauv. Apata J. T, Ogunleye S.G, Ogunbiyi O. J, Babalola O.O. IJBST (2017), 10(11):80-88.
FULL TEXT SWF:
GC-MS Analysis and Phytochemical Screening of n-Hexane Fraction Constituents from the Leaf of Clerodendrum volubile P. Beauv.
Authors & Affiliation:
Apata J. T1, Ogunleye S.G2, Ogunbiyi O. J3, Babalola O.O.4
1,2,4 Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
3 Department of Biochemistry, Faculty of Life Science, University of Benin, Edo State, Nigeria
The study investigated the bioactive principles from n-hexane fraction of the leaf of Clerodendrum volubile using phytochemical screening test and GC-MS techniques. The leaves of C. volubile plant material were harvested, air-dried for 72 h. The dried leaves were milled into powdered form using electric blender. Powdered plant material (60 g) was suspended in 3 L of absolute methanol for 48 h at 25oC. The suspension was filtered through a double layer cheese-cloth. The extraction process was repeated five times until the extract became clear. The filtrates were pooled and concentrated under reduced pressure at 35ºC to give dark green residue. The total crude methanolic extract was stored in the desiccator until it was required for further processing. In GC-MS analysis, identification of the constituents was carried out by comparing the mass spectrum fragmentation pattern of each of the constituents with those stored in the data base of National Institute Standard and Technology 11 (NIST11.L) library. The results of the phytochemical screening gave a positive reaction for the presence of flavonoids, alkaloids, resins, tannins and phenols while glycosides, saponins, phlobatannins, sterols, carbohydrates and terpenoids were absent. Also, the GC-MS analysis of the leaf of the plant revealed the presence of six (6) bioactive compounds out of which three compounds were prominent. The prominent constituents of the plant were bicyclo [3.1.1] heptane, 2, 6, 6 –trimethyl-, 1-Hexadecyne and dodeca -1, 6 – dien – 12 – ol, 6, 10 – dimethyl. The study therefore concluded that the bioactive compounds identified in the leaf of C. volubile may be responsible for the ethnomedical or traditional use of this plant in the treatment and management of various diseases and also, it could be a plausible explanation for the reported biological activities exhibited by this plant. Hence, Clerodendrum volubile leaves could serve as a source for these bioactive principles which may be employed as ingredients for the formulation of novel drug.
KEYWORDS: GC-MS analysis, Phytochemical Screening, Clerodendrum volubile, Bioactive Principles.