IJBST 2017 Volume 10 Issue 5

International Journal of BioSciences and Technology (IJBST) ISSN: 0974-3987

An Open Access Journal -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives



Title:
Biomass and genetic variability among Telfaira occidentalis, Celosia argentea and Talinum triangulare harvested from Okitipupa, Nigeria studied by random amplified polymorphic DNA (RAPD)

Authors & Affiliation:
B.K. Bello, F. Adetuyi, O. Oluwaseun, O. Anthonia and S. Adegoke
Department of chemical sciences, Faculty of science, Ondo State University of Science and Technology, Okitipupa, Nigeria

Abstract:
Random Amplified Polymorphic DNA (RAPD) technique can be considered to be an important tool in phylogenetic studies, species identification, and assessment of genetic variability. In the present study, a “RAPD” technique was utilized to detect genetic variability among Telfairia occidentalis, Celosia argentea and Talinum triangulare harvested from selected locations in Okitipupa metropolis. A total sum of 47 bands was obtained in all the samples of Telfaira occidentalis amplified using the 5 RAPD arbitrary primers, for Celosia argentea a sum total of 26 bands was obtained in all the samples amplified, while a total sum of 61 bands was obtained in all the samples of Talinum triangulare amplified. The bands obtained ranged in size from 100 - 1200 bp. The extinction ratio (260/280 nm) of the isolated DNA was in the range from 1.5 to 2.5, indicating that the DNA was pure enough for RAPD analysis. Investigation of the plant biomass displayed evidence of reduced biomass accumulation. The results of this study suggested that RAPD technique can be used to detect polymorphism and genetic biodiversity in plant species. 

Key words: Telfaira occidentalis, polymorphism, RAPD, Celosia argentea and Talinum triangulare

Ċ
Prof. Dr. Prabhu Britto Albert,
Sep 26, 2017, 6:28 AM
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Prof. Dr. Prabhu Britto Albert,
Sep 26, 2017, 6:28 AM